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Ornamental Fish

Pterophyllum scalare
螢光神仙魚
Gene Transfer in Taiwan is World-Renowned
Internationally, Taiwan is a leader in biotechnology research and development. Today (May 25), a press conference at the Council of Agriculture, Executive Yuan, was held by scientists and the Jy Lin Company , the biggest exporter of ornamental fish in Taiwan, to announce that the first green-fluorescent angelfish (Pterophyllum scalare) and convict cichlid (Cichlasoma nigrofasciatus) in the world have successfully been developed. The body colors of these two fish are a beautiful green fluorescent under blue light (as shown in the picture). With help from the Council of Agriculture and Fishery Agency, this industrial-academic cooperation received technical guidance from Academia Sinica and the “Office for the Promotion of Industrialization of Agricultural Biotechnology”. Then, through the screening and breeding undertaken by the technical team at Jy Lin Company, the beautiful new fish were cultivated which can be used for ornamental, scientific, and medical applications.

According to Yu-Ho Lin, Chairman of Jy Lin Company, these ornamental fish are aquaculture species with high economic value and great development potential. They are very suitable for development in Taiwan which is experiencing rising manpower costs and limited water resources at present. In addition to cats and dogs, ornamental fish are also very popular pets, due to the great variety of breeds and the various gorgeous colors. To develop the ornamental aquarium industry, two useful biotechnology tools of gene engineering and gene transfer can be utilized to change fish species and produce special genetic strains; for example, increasing or improving the appearance of the body color to make new kinds of tropical fish with a variety of hues. Through these techniques, we can greatly enhance a fish’s ornamental value and industrial competitiveness in international markets.

According to Dr. Jyh-Yih Chen of the Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, proper breeds and improvements are a crucial influence in terms of developing biotech pets with high economic value. After discussions with Jy Lin Company, he chose the common angelfish and convict cichlid to be the research subjects. He also suggested that Jy Lin Company speed up the cultivation of transparent strains of these two fishes, with the expectation that the implanted fluorescent protein genes would fully perform.

Three years ago, Academia Sinica began research on muscle promoters. Researchers recently found a new type of promoter with a whole-body muscle performance gene. A plasmid was also constructed, by means of a promoter connected to the green fluorescence protein gene and an antimicrobial peptide gene. The first convict cichlid implanted with the improved muscle-promoter fluorescence protein gene was born in the Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, on August 15, 2008. Through the technique of marker-assisted selection (MAS) provided by the Jy Lin Company, the molecular markers were applied to genetic breeding and improvement. To the present, the third generation (F3) has successfully been born, which has established a solid foundation for the industrialization of the achievements in such transgenic ornamental fish. “In the world, this is the first instance in which the fish of the Family Cichlidae have been implanted with the green fluorescent gene and have successfully passed it down to the next generation.” Undoubtedly, such research is a new milestone in terms of biotech pets. This is not only the first instance of fluorescent ornamental fish of the Family Cichlidae in the world, but also the largest fluorescent ornamental fish worldwide at the present stage! Such an achievement has initiated inspiring prospects of research on high-class ornamental fish.

Yu-Ho Lin pointed out that the common angelfish is a beautiful and elegant ornamental fish from the Amazon River. It was a global sensation when it was introduced to the international market in 1915. A pair of them might cost up to US$75. At that time, a new car cost only US$500. Because the common angelfish has graceful fin lines and a moderate personality, it still remains one of the most popular ornamental fish to this day. The transparent common angelfish implanted with the fluorescent protein gene is a breed exclusively cultivated by Jy Lin Company: its skin is transparent, while its internal organs can clearly be seen. Among the common angelfish from the Amazon River, the research personnel at Jy Lin Company searched for individuals with lighter skin color due to gene mutations, and bred them to produce later generations. Then, among the later generations, the ones with the lightest color were once again selected for breeding. After similar selection and breeding were repeatedly undertaken, individuals of the common angelfish with an almost-transparent skin color were finally cultivated.

Yu-Ho Lin said: The R&D team at Jy Lin Company adopted the transfer method via reproductive ovary electroporation, to transplant the external fluorescent protein gene into the transparent common angelfish’s ovaries. The first batch of F0 transgenic common angelfish (96 in total) were obtained in January 2008. After cultivating approximately 20,000 individuals, the research personnel were able to obtain transgenic fish that exhibited stable performance and passed the trait on to subsequent generations—the fluorescent common angelfish shows green fluorescent light from the inside out. Through two kinds of authentication via molecular biological techniques, the results were completely consistent, which proved that gene transfer was successful.

With technical guidance provided by the Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica for many years, the Jy Lin Company has accumulated considerable experience in technical aspects of gene transfer in ornamental fish; these techniques are widely applied to many kinds of popular-selling fish internationally. At present, this industrial-academic cooperation is still actively ongoing, and aims to successfully apply a sterility technique to transgenic ornamental fish. In addition, by means of marker-assisted selection (MAS) in fish to analyze genetic variations and formation, the speed and efficiency of breeding fluorescent ornamental fish can be enhanced, so as to advance commercial mass production. In the meantime, Jy Lin Company also hopes as soon as possible to get these fish approved under the genetically modified organism (GMO) laws in various countries. In particular, they hope to gain approval under the Taiwanese GMO law.

When the Jy Lin Company carried out the research and developed the first green fluorescent common angelfish and convict cichlid in the world, many foreign biological museums actively invited the company through many channels to go there to exhibit these first green fluorescent species in the world.

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蔬菜對溫度日照條件的要求

蔬菜對溫度日照條件的要求
全日照  8個小時日照 瓜類、茄果類、豆類、山藥、豆薯(地瓜)。番茄、黃瓜、茄子、辣椒等喜溫中、強光性
蔬菜夏秋季生產,玉米、青椒、西瓜、南瓜、西紅柿、茄子、芝麻、向日葵類。
其次是根莖類,如:馬鈴薯、甜菜、胡蘿蔔、白蘿蔔、甘藷、山藥等等。至少需半日照,才能生長,芋頭雖喜歡全日照,但比其他蔬菜耐蔭。 
需要中等光照大白菜、甘藍、芥菜、蒜、洋蔥。 

長日性蔬菜白菜、甘藍、芥菜、蘿蔔、胡蘿蔔、芹菜、菠菜、萵苣、蠶豆、豌豆、大蔥、洋蔥。

短日性蔬菜豇豆、扁豆、莧菜、空心菜。         

中光性蔬菜黃瓜、番茄、茄子、辣椒、菜豆

菜豆

菜豆喜溫暖,不耐高溫和霜凍。菜豆種子發芽的適溫為20-30℃;在40℃以上的高溫和10℃以下的低溫,種子不易發芽。幼苗生長適宜氣溫為18-25℃。花芽分化的適宜氣溫為20-25℃,過高或過低溫度易出現發育不完全的花蕾、落花。

菜豆對光照強度的要求較高。在適宜溫度條件下,光照充足則植株生長健壯,莖的節間短而分枝多,開花結莢比較多,而且有利於根部對磷肥的吸收。當光照強度減弱時,植株易徒長,莖的節間長,分枝少,葉質薄,而且開花結莢數少,易落花落莢。

菜豆根系強大,能耐一定程度乾旱,但喜中度濕潤土壤條件,要求水分供應適中,不耐澇。生長期適宜土壤濕度為田間最大持水量的60%-70%,空氣相對濕度以80%為宜。開花結莢期對水分最敏感,此期土壤乾旱對開花結莢有不良影響,開花數、結莢數及莢內種子數減少。土壤水分過大時,下部葉片黃化,早脫落。空氣濕度過大會引起徒長、結莢不良。

菜豆具有深根性和根瘤菌,對土壤的要求不甚嚴格,但仍以土層深厚肥沃、排水良好的輕砂壤土或粘質壤土為好。土壤過於粘重、低溫、排水和通氣不良則生長不良,炭疽病重。菜豆喜中性至微酸性土壤,適宜的土壤pH為5-7.0,其中以州6.2-6.8最適宜。菜豆最忌連作,生產中應實行2-3年輪作。

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蝶豆花

蝶豆花 原產拉丁美洲的蝶豆花是一種典型的熱帶蔓藤植物,全年盛開。
butterfly pea,拉丁語叫:Clitoria ternatea,泰語叫Dok Anchan
中文名叫蝶豆花,藍蝶花,藍蝴蝶、蝴蝶藍花,還有蝴羊豆、豆碧等別名。
用蝶豆花當作高品位浪漫的茶品飲用、以及當作天然食品色素制作糕點是拉丁美洲和南洋國家的風情和習俗。
蝶豆花的味道自然甘甜,南洋國家的一些五星級酒店通常把蝶豆花茶當作高貴的迎賓茶來接待貴賓。
營養價值 蝶豆花具有豐富的維他命A,C和E, 而且可以提高免疫力, 幫助和促進皮膚的彈力和骨膠原, 同時還具有補腦,促進腦的活力,防止胃痛,抗憂郁、抗壓力、鎮靜、止驚厥、緩和情緒等天然保健功效。
蝶豆花中的天然藍色素,也是有療效的。如果將其加入檸檬並調制成花茶飲品,就是保健心臟血管的絕佳飲料。
食用價值 蝶豆花的可食部位是葉、花及嫩莢。較幼嫩的葉片及盛開的花朵,亦可拿來煮湯、油炸等。用嫩芽來炒肉絲或煮熟後食用,都十分可口。蝶豆花的葉及花的萃取液,可當作純天然的食品染料。

直達香草(herb4kitchen)
PS.營業用批量報價

錦鯉養殖基本知識

◎飼養與管理的重點 只要不是劇烈的變化,錦鯉很容易適應各水溫水質等環境的變化。並不是沒有大庭園就無法飼養,有人甚至在二樓陽台或頂樓陽台造水池飼養。然而我們是欣賞錦鯉雄壯豪邁之氣,因此水池盡量寬闊為宜,以水深1.2m以上為理想。魚池必須有底水排出,過濾循環等設備。用水不一定要取地下水,自來水也可以飼養。
<因為都市中有景觀安全的需求,及屋頂花園有荷重的需求,錦鯉池水深可以低到30cm左右。>
◎每天排水
A、糞或枯死的藻類全部送至過濾槽的話,耗氧量會增大,pH就下降,更會轉變為亞硝酸,增了過濾槽的負擔。為了盡量減輕過濾槽的負擔,每天至少把魚池的底水排水使固物排出去,把中間水送去沉澱槽及過濾槽。 
B、把固體廢物的魚糞集中排出,最好不要從池底打氣而是從排糞口的上方40~50公分打打氣。如此氣泡往上昇。池水產生對流。污物就集中於排糞口。
<可以設計水流把固體廢物盡量集中或排出到過濾系統中。>
◎過濾槽管理
A、細菌附著於濾材,分解固體廢物會消耗大量的氧。 
B、溶氧不足時,厭氧菌會把硝酸還原亞硝酸,或從碳酸氣發生沼氣,也會從硫酸分解產生硫化氫等有毒氣體。
<如果溶氧不足,可以優先把打氣設備放置到生化過濾槽中。>
◎溶氧要充份
A、水中溶氧不足的話,會影響錦鯉的生育,飼料的消化,,水質的維持等等。
B、硝酸,亞硝酸的濃度增高時,會影響溶氧量。所以優先去除硝酸及亞硝酸。
C、使用沸石可輕易去除硝酸,沸石量約等重於魚體總重量。
<沸石再生法,是將沸石浸泡25℃以上1:10食鹽水數小時,再以清水洗淨即可。>
◎水質的控制
水質硬度高的話,錦鯉肌膚經常會有少許充血的狀態。豔麗性也會慢慢消失,紅緋會上升。pH值低,肌膚變的很不好看,但是雖餵增色飼料,依然不見起色,徒增浪費。pH值7.1~7.5最適宜的。
◎鯉餌的重要性
良好的魚餌不會崩壞鯉的體型。餌的量也是在夏天水溫 高的時候,訂定停餌期間,才是整體來說使鯉變胖最重要的秘訣。如果還是想 要給很多餌的話,要增加循還量。錦鯉在水溫超過28度的時候,應給與相當於 鯉全體重量3%的餌。水溫25度時1.5%,水溫20度時0.3%,16度以下則要停止鯉餌,這就是鯉魚長得強壯的要訣。連續不斷地給鯉餌的話,引起內臟障礙, 而影響到鯉不會長壯,甚至導至體型的變歪。
◎魚病預防
水的管理與定期消毒都是很重要的步驟,…